Get your European, Iberian disease away from my children, said the Emperor of the Inca Empire! Western Coast of Chile, 1499.
Shots rang out, the firing line behind the first line was preparing their musket ball munitions to advance forward. After the first day of the Spanish Army attacking 4 square miles of the central most portions of the Inca Empire, not one arms-men, woman, or child of that 4 square mile area native to the Inca Empire survived.
Meanwhile, 8,000 miles away at the Cape of Good Hope, Portuguese guardsmen were instructed by Queen Elizabeth to start building another camp.